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(m) DNA damage foci in senescent MAFs of the indicated genotype (left, shown by 53BP1 immunofluorescence in red) and in co cultured reporter fibroblasts (right, foci also contain green 53BP1 GFP fusion protein). (n) Senescent nfkb1/ MAFs induce more DNA damage in non senescent bystander cells than senescent wt MAFs. 53BP1 GFP foci frequencies in bystander fibroblasts after the indicated times of co culture are shown..
ConclusionIn this study, high throughput sequencing of the bacterial 16S rRNA gene combined with clone libraries (amoA, nirS and nirK) were applied to investigate nitrogen cycling bacterial communities in activated sludge. Community structure analysis revealed that a core set of bacteria existed in all samples. Phylogenetic analysis of ammonia oxidizing and nitrite reducing bacteria showed that Nitrosomonas, Thauera and Mesorhizobium were the most dominant functional communities involved in nitrogen removal.
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